Journal: The Journal of Neuroscience
Article Title: The Second Galanin Receptor GalR2 Plays a Key Role in Neurite Outgrowth from Adult Sensory Neurons
doi: 10.1523/JNEUROSCI.23-02-00416.2003
Figure Lengend Snippet: A, The percentage of cells bearing neurites from dissociated DRG cultures isolated from wild-type and galanin knock-out animals in the presence and absence of 100 nm AR-M961 or AR-M1896 8 hr after plating. The addition of either AR-M961 or AR-M1896 rescued the deficits in percentages of cells producing neurites seen in galanin knock-out cultures to near wild-type levels. Addition of AR-M1896 to wild-type cultures significantly increased the percentage of cells bearing neurites compared with controls. Although addition of AR-M961 increased the percentage, this was not significant. B, The length of neurite outgrowth from dissociated DRG cultures isolated from wild-type animals in the presence and absence of 100 nm AR-M961 or AR-M1896 8 hr after plating. Addition of either AR-M961 or AR-M1896 significantly increased neurite length. C, The percentage of cells bearing neurites from dissociated DRG cultures isolated from wild-type and galanin knock-out animals in the presence and absence of 10 μm BIM or 10 μm BIM plus AR-M1896. Significant deficits are seen in the number of cells producing neurites in wild-type cultures in the presence of 10 μm BIM, which was not rescued by the addition of 100 nm AR-M1896. Addition of 10 μm BIM had no effect on mutant cultures. Data are presented as percentage of cells bearing neurites or mean ± SEM length (t test; *p < 0.05; ***p < 0.001; n= 5).
Article Snippet: Cells were cultured in DMEM–F12-supplemented medium as described above with or without the addition of the following chemicals: 1 n m M35 [galanin(1–13)bradykinin(2–9)] (Bachem UK, Essex, UK), 100 n m galanin peptide (Bachem UK), 10 μ m bisindolylmaleimide I (BIM) (Calbiochem, La Jolla, CA), 10 n m RWJ-57408 [2,3-dihydro-2-(4-methyl-phenyl)-1,4-dithiepine-1,1,4,4-tetroxide] (Johnson & Johnson, Spring House, PA), and 100 n m AR-M961 ([Sar(1), d -Ala 12 ]Gal(1–16)-NH 2 ) or AR-M1896 [Gal(2–11)Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-NH 2 ] (AstraZeneca, Montreal, Quebec, Canada).
Techniques: Isolation, Knock-Out, Mutagenesis
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